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. 2005 Feb 18;102(9):3447–3452. doi: 10.1073/pnas.0407759102

Fig. 3.

Fig. 3.

Inducible DNA-binding activity of the transcription factor Egr-1 (Egr-1) in C. pneumoniae (Cp)-infected CASMCs is first seen after 4 h (lane 4). Besides the inducible Egr-1 signal, constitutive DNA binding (C) is present after 1 and 4 h (lanes 1–4). Competition experiments with a 50-fold excess of unlabeled cold probe (lanes 5–8) but not of control probe (lanes 13–16) prevented DNA–protein interaction of both constitutive and inducible DNA binding. Supershifts with anti-Egr-1 antibody proved specificity of the inducible DNA binding (lanes 9–12). The data represent three independent experiments. SS, supershifted signal.