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. 2017 Jul 26;12(7):e0181804. doi: 10.1371/journal.pone.0181804

Fig 2. Binding of ESC and SOB3 to the YUC9 promoter.

Fig 2

(A) Putative S/MAR region in the YUC9 promoter. The predicted S/MAR region is marked with an arrowhead. Underbars represent the amplified genomic regions. (B and C) ChIP assays. Total protein extracts from 35S:ESC-MYC (B) and 35S:SOB3-MYC (C) transgenic plants grown for 9 days under LD conditions were immunoprecipitated with an anti-MYC antibody. Fragmented DNA was eluted from the protein-DNA complexes and used for qPCR analysis. Three independent biological replicates were averaged, and the statistical significance of the measurements was determined by two-tailed Student's t-test assuming unequal variance (*P < 0.05). Bars indicate the standard error of the mean.