Figure 3.

Tobacco BI-1 and ATG6 interacted at the ER. nYFP and cYFP are the N- and C-terminal domains of YFP, respectively. BI-1 was fused to the N-terminal end of nYFP. ATG6 was fused to the C-terminal end of cYFP. Yellow puncta in the first column indicate interactions between ATG6 and BI-1 and colocalize with puncta (first row and second row, arrows) labeled with the ER marker, ER-cb. No interaction was detected in the BI-1-nYFP+cYFP-nLUC negative control (third row and fourth row). Experiments were repeated 3 times.