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Unnecessary residues removed, and sequences shuffled. Direct removal of Gly9 and Phe15 from the ligand 6 sequence to create ligand 6(ΔGF) decreased binding. When the sequence lacking Gly and Phe is shuffled, however, many library members retain or increase binding ability. The 7-mer shuffled sequence (indicated by asterisk) with the highest binding was used for subsequent studies.
