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. Author manuscript; available in PMC: 2017 Jul 26.
Published in final edited form as: J Med Chem. 2016 Apr 11;59(8):4019–4025. doi: 10.1021/acs.jmedchem.5b01536

Figure 5.

Figure 5

Demonstration of selectivity. CAV(1-104) and several control proteins were labeled with rhodamine and screened against duplicate SPOT sheets containing ligands 5, 6, and 1 to demonstrate selectivity of the ligand. Bovine serum albumin (BSA), casein, and hen egg white lysozyme (HEWL) all bound ligand 1 with reduced apparent affinity relative to CAV(1-104) as measured by fluorescence. Blank SPOTs containing only the double β-Ala linker were included as negative controls, and these baseline signals were subtracted from the corresponding signal for ligands 5, 6 and 1 SPOTs on each sheet.