Table 2. SAGE libraries.
| Normalized SAGE tag counts
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Monocyte
|
Non-monocyte
|
||||||||
| Gene description | SAGE tag sequence | Unigene ID | P1 | P2 | C1 | A1 | A2 | P1 | A1 |
| Hematopoietic markers | |||||||||
| Monocyte | |||||||||
| CD14 antigen | TGGTCCAGCGCCCTGAA | 163867 | 47 | 64 | 100 | 106 | 78 | 4 | 0 |
| CD163 antigen | GAGGTTCCTGGGGGACA | 504641 | 23 | 20 | 21 | 11 | 20 | 0 | 0 |
| Non-monocyte | |||||||||
| CD3E antigen, epsilon (TiT3 complex) | TAAGTTGTCCCCCATCC | 3003 | 0 | 0 | 0 | 0 | 5 | 28 | 54 |
| CD79A antigen (Ig-associated alpha) | TATGAGGACATCTCCCG | 79630 | 0 | 2 | 2 | 2 | 2 | 30 | 20 |
| Pan-leukocyte | |||||||||
| CD99 antigen | GGATGTGAAAGGCTGGC | 495605 | 26 | 48 | 32 | 29 | 39 | 66 | 54 |
| Monocyte candidate genes | |||||||||
| FOS, osteosarcoma viral oncogene homolog | TGGAAAGTGAATTTGAA | 25647 | 111 (5.8) | 138 (7.3) | 19 | 27 | 11 | 8 | 4 |
| DUSP1, dual specificity phosphatase 1 | CTTGACATACCTACCAG | 171695 | 71 (3.0) | 92 (3.8) | 24 | 17 | 14 | 12 | 2 |
| NFKBIA, NFK gene in B cell inhibitor, alpha | TAACAGCCAGGAGTGCT | 81328 | 40 (2.0) | 56 (2.8) | 20 | 10 | 15 | 16 | 16 |
| ID2, inhibitor of DNA binding 2 | CTAAACTTTTTATAAAA | 180919 | 32 (1.7) | 42 (2.2) | 19 | 7 | 5 | 16 | 12 |
| PER1, period homolog 1 | GAGTCCCTGGTGCTGCC | 445534 | 30 (1.8) | 60 (3.5) | 17 | 1 | 1 | 0 | 2 |
| SAP30, sin3-associated polypeptide, 30 kDa | TAGAAATGTTCTTTGTG | 512813 | 10 (1.7) | 30 (5.0) | 6 | 3 | 4 | 2 | 0 |
Hematopoietic markers and monocyte candidate gene tag counts are tabulated under the various SAGE libraries, along with their associated sequences and gene identification numbers. A total of seven SAGE libraries are shown, five CD14+ monocyte (Monocyte) and two monocyte-depleted (Non-monocyte) libraries. The tag counts shown are normalized to 100,000 tags per library. Parentheses represent patient-to-control C1 tag ratio. P1, patient 1; P2, patient 2; C1, age-matched control 1; A1, younger age control 1; A2, younger age control 2.