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Purified Nbp35p is an Fe/S protein. UV-vis spectrum of N-terminally His-tagged Nbp35p (1.9 mg/ml/50 mM Tris·HCl, pH 8/1 μM 5′-deazaflavin) before (OX) and after (DAF) photoreduction by irradiation for 3 min with a commercial slide projector. The contribution of oxidized 5′-deazaflavin to the spectrum before photoreduction has been subtracted. Inset shows the results of colorimetric iron and acid-labile sulfide determinations for five independent samples.
