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. 2005 Feb 22;102(9):3266–3271. doi: 10.1073/pnas.0406447102

Table 1. Enzymatic activities upon depletion of Nbp35p in Gal-NBP35 cells.

Wild type
Gal-NBP35
Compartment Gal Glc Gal Glc
Cytosol
   Isopropyl malate isomerase 0.30 ± 0.01 0.31 ± 0.05 0.40 ± 0.09 <0.04
   Alcohol dehydrogenase 1.8 ± 0.3 2.0 ± 0.3 1.6 ± 0.2 2.4 ± 0.6
Mitochondria
   Aconitase 3.5 ± 0.9 3.2 ± 0.4 2.9 ± 0.5 3.7 ± 0.6
   Succinate dehydrogenase 0.30 ± 0.03 0.20 ± 0.02 0.20 ± 0.01 0.30 ± 0.01
   Malate dehydrogenase 2.0 ± 0.1 1.5 ± 0.1 1.6 ± 0.1 1.5 ± 0.1
   Citrate synthase 2.8 ± 0.4 2.9 ± 0.3 3.1 ± 0.6 3.7 ± 0.5

Enzyme activities of cytosolic and mitochondrial proteins were determined using postmitochondrial supernatants and mitochondria, respectively, isolated from wild-type and Gal-NBP35 cells grown in minimal media containing galactose (Gal) or glucose (Glc). Activities are given in units per mg of protein.