Table 1. Enzymatic activities upon depletion of Nbp35p in Gal-NBP35 cells.
| Wild type
|
Gal-NBP35
|
|||
|---|---|---|---|---|
| Compartment | Gal | Glc | Gal | Glc |
| Cytosol | ||||
| Isopropyl malate isomerase | 0.30 ± 0.01 | 0.31 ± 0.05 | 0.40 ± 0.09 | <0.04 |
| Alcohol dehydrogenase | 1.8 ± 0.3 | 2.0 ± 0.3 | 1.6 ± 0.2 | 2.4 ± 0.6 |
| Mitochondria | ||||
| Aconitase | 3.5 ± 0.9 | 3.2 ± 0.4 | 2.9 ± 0.5 | 3.7 ± 0.6 |
| Succinate dehydrogenase | 0.30 ± 0.03 | 0.20 ± 0.02 | 0.20 ± 0.01 | 0.30 ± 0.01 |
| Malate dehydrogenase | 2.0 ± 0.1 | 1.5 ± 0.1 | 1.6 ± 0.1 | 1.5 ± 0.1 |
| Citrate synthase | 2.8 ± 0.4 | 2.9 ± 0.3 | 3.1 ± 0.6 | 3.7 ± 0.5 |
Enzyme activities of cytosolic and mitochondrial proteins were determined using postmitochondrial supernatants and mitochondria, respectively, isolated from wild-type and Gal-NBP35 cells grown in minimal media containing galactose (Gal) or glucose (Glc). Activities are given in units per mg of protein.