Table 1. Doubling times (in minutes) of an rpoS strain in different backgrounds on poor N and S sources.
| Strain | Genotype | ![]() |
Arginine
|
glutathione |
taurine |
Hepes |
lanthionine |
|---|---|---|---|---|---|---|---|
| NCM3964 | zih-102* | 60 | 230 | 120 | 70 | 70 | 55 |
| NCM3890 | rpoS* | 60 | 180 | 180 | 80 | 80 | 70 |
| NCM3963 | zih-102† | 75 | 630 | 90 | |||
| NCM3962 | rpoS† | 70 | 260 | 200 |
Cells were grown in N-C- or N-C-S- minimal medium with glycerol (0.4%) as the C source. The N sources (10 mM total N) and S sources (0.25 mM total S) were as indicated. Doubling times with glucose (0.2%) [or lactose (0.2%)] were: 50 min for NCM3964 and NCM3890 and 65 and 60 min, respectively, for NCM3963 and NCM3962.
The strain carries a Tn10 insertion in the background of NCM3722
The strain carries a Tn10 insertion in the background of MG1655 (CGSC 6300) [also known as NCM3105 (5)]





