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. 2005 Feb 18;102(9):3383–3388. doi: 10.1073/pnas.0407488102

Fig. 5.

Fig. 5.

Recognition of KRN7000, C24:0, and C20:2 by the same population of Vα14i NKT cells. (A) Costaining of C57BL/6 splenocytes or thymocytes with allophycocyanin-conjugated CD1d tetramers assembled with C24:0, and phycoerythrin-labeled CD1d tetramers assembled with various analogues. (B) Thymocytes were stained with C24:0, C20:2, KRN7000, or vehicle-loaded CD1d tetramers–phycoerythrin, and with antibodies to B220, CD3ε,Vβ7, Vβ8.1/8.2, or NK1.1. Dot plots show gating for tetramer+ T cells, after exclusion of B lymphocytes, and dead cells. (C) TCR Vβ and NK1.1 phenotype of tetramer+ CD3εint thymocytes. Analogous results were obtained with splenocytes. The results shown are representative of three or more experiments.