Figure 4d:

Histologic analyses, MR imaging, and elastography results in mice with NAFLD or NASH. (a, b) Histologic images stained with hematoxylin-eosin (H&E ) or sirius red (a) and MR elastography–assessed liver stiffness (80 Hz) and damping ratio (80 Hz) maps (b) in selected mice with NAFLD or NASH on 1, 24, and 48 weeks of a fast-food diet. Livers are delineated with dotted lines. Locations of the vibrating needle (yellow ○) were excluded from calculation. (c–e) Graphs show steatosis and weight analyses (c and f ), inflammation analyses of cellular injury and fibrosis (d), and alanine aminotransferase analyses (e) of the mice with NAFLD or NASH. At each individual frequency (80–200 Hz), comparisons of MR elastography parameters were performed between mice with NASH and control mice at every different feeding time from 1 week to 48 weeks (selected time points and frequencies are given in Appendix E1 [online]). (g, h) To simplify graphic illustration, liver stiffness (g) and damping ratio (h) were normalized with regard to the same age control groups (ie, means in animals with disease were divided by means in control animals). All comparisons were performed with Welch t tests between the disease and control subgroups at different frequencies prior to normalization. Significant increases (red arrow) and decreases (blue arrow) are indicated for each comparison. The number of animals (animals with disease vs control animals) is also indicated in gray at the bottom of the horizontal axis in e and h. Data for control animals are not shown in all bar plots.