Table 2.
Primers pairs used for qPCR.
| Primer | GenBank accession number | Primer sequence (5′ to 3′) | Primer concentration (nM) | Product size (bp) |
|---|---|---|---|---|
| Forward/reverse | ||||
| KLF2 | NM_001318423 | GCTTCTACCAGACAAACCCG | 250 | 233 |
| CAGGACTGGCCCATAACTGT | ||||
| EDN1 | XM_418943.4 | GATGTGCCAGCCAGAGAGACAA | 375 | 180 |
| CAGCCTCCAGCCTCTTCATTTTC | ||||
| TBX20 | NM_204144.1 | AGATATGCCTACCACCGCTCCT | 375 | 178 |
| TGATGTGGCCATGCTGATCCA | ||||
| Aggrecan | NM_204955.2 | CGGCATCTGGACAAGAGACAGA | 250 | 165 |
| CTCCATTCAGACAGGGGCTTGA | ||||
| Periostin | NM_001030541 | AGAACCTGATCTCATGGCAACCA | 250 | 183 |
| GGGTTGTAAAACGTCAGTGGAATCT | ||||
| TGM2 | NM_205448.1 | CCAGCCCCACATGGAACAGA | 375 | 128 |
| CCACGCTGTCACCAGTCTCA | ||||
| GAPDH | NM_204305.1 | AGACGGTGGATGGCCCCTCT | 375 | 263 |
| ACGGCAGGTCAGGTCAACAACA | ||||
| EEF1A1 | NM_001321516.1 | GCTCTAACATGCCCTGGTTCAAG | 375 | 188 |
| TGGCTTCAGGACACCAGTTTC | ||||
| FBN2 | XM_004949379.2 | GCCCATGTGAGCGGTGTGAA | 250 | 194 |
| CACTCGCCCTGTTCCATCCA | ||||
| COL3A1 | NM_205380 | CTGGAAGGGCAGGGAACAAC | 250 | 249 |
| GGCATGGCTCTGGTTTCCAA | ||||
| TNC | NM_205456.4 | GCTGAGGGTGGATGGCTACAG | 250 | 168 |
| CCCATCAGATTGACTCGGTGACA |