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. 2017 Jul;108:1–7. doi: 10.1016/j.yjmcc.2017.05.003

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© 2017 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 1 — Application of the V:SA method to TTs in rabbit ventricular myocytes. (A) Shows a region of a myocyte dual labeled with FM4-64 for the surface membrane (left panel), and calcein for the extracellular space -which includes the TT lumen (right panel). (B) Shows the same optical sections after processing (background subtraction, PSF modification to a sphere and normalization). (C) The left panel shows the V:SA values calculated for pixels within the TT mask. The right panel shows a magnified view of the region marked in C (dashed white box), where the TT skeleton was dilated to reflect the calculated TT width and the color table indicates V:SA. Scale bars show 10 μm. (D) A histogram of V:SA values within the TT skeleton from the cell shown in A-C. The lower scale bar shows conversion from V:SA to TT diameter. Means of all data are given in Table 1.