Fig. 3.

Application of the V:SA method to TTs in mouse ventricular myocytes. (A) Shows a region of a myocyte co-labeled with FM4-64 for the surface membranes (left panel), and calcein for the extracellular space and TT lumen (right panel). (B) Shows the same optical sections after image processing as described for Fig. 1B. (C) The left panel shows the V:SA values calculated for pixels within the intermediary TT mask. The right panel shows the region marked in C (dashed white box). Scale bars show 10 μm. (D) Shows histograms of V:SA values within the TT skeleton from the cell shown in A-C. The lower scale bar shows conversion from V:SA to TT diameter. Mean data is given in Table 1.