Skip to main content
. Author manuscript; available in PMC: 2017 Jul 27.
Published in final edited form as: Leukemia. 2016 Dec 28;31(8):1798–1807. doi: 10.1038/leu.2016.392

Figure 3. The GIMAP enhancer is activated in normal zebrafish HSCs.

Figure 3

(A) Schematic representation of the experimental design. The GIMAP enhancer (eG) was introduced into the reporter construct carrying a minimal promoter sequence (promoter) and an eGFP gene. The RUNX1 stem cell enhancer (eR1) was cloned into the reporter construct encoding the DsRed gene. The reporter constructs were injected into a single-cell zebrafish embryo. The cartoon displays regions of zebrafish hematopoietic tissues. The kidney marrow (KM), aorta-gonad-mesonephros (AGM), caudal hematopoietic tissue (CHT), and thymus (T) are indicated. (B) Activation of the reporter constructs in early hematopoiesis in zebrafish. Fluorescent images show one representative zebrafish embryo (n=16) injected with reporter constructs at 2 and 3 dpf. The AGM and CHT are indicated by white and red arrowheads, respectively. Scale bar, 1 mm.