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. Author manuscript; available in PMC: 2017 Jul 27.
Published in final edited form as: Virology. 2017 Apr 28;507:242–256. doi: 10.1016/j.virol.2017.04.008

Fig. 3. Mouse immunization schedule.

Fig. 3

(A) Six-week-old C57BL/6 mice were immunized at days 0, 45, and 90 with different combinations of recombinant vaccinia virus and plant-produced HIV-1 VLPs, represented as an image of a poxvirus or green-enveloped particles, respectively. Vaccinia virus doses (VV) consisted of 1×106 pfu of equally mixed NYVAC-KC-Gag and NYVAC-KC-dgp41 delivered i.m. while plant-produced VLPs were mixed with Ribi adjuvant and delivered i.p. with 2 μg p24 and 1.2 μg MPER. Serum, fecal, and vaginal lavage samples were collected once every 2 weeks (indicated by arrows). Serum samples were analyzed for antigen-specific IgG to p24 Gag (B), or the MPER region of dgp41 (C), shown over the length of the experiment as the ELISA OD 490 nm for the 1:50 dilution (lowest tested).