Table 1.
Summary of Optimal Kinetic Values Obtained in the Absence of Profilin by Fitting with Eq. 1
| ATP | ADP | |
|---|---|---|
| kon (μM−1 s−1) | 21 ± 0.40 | 3.6 ± 0.04 |
| koff (s−1) | 1.3 ± 1.1 | 3.9 ± 1.3 |
| P0c (p0depoly) | 0.29 ± 0.04 | 0.61 ± 0.04 |
Values were obtained by global fit of the low- and high-tension experiment elongation rates shown in Fig. 3, A and B, with Eqs. 1 and 2′, and the values correspond to the fitting curves (straight lines) in these figures. Fitting was performed using the software Origin (OriginLab, Northampton, MA). Values given are mean ± SE.