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. 2017 Jul 26;9(7):98–106. doi: 10.4252/wjsc.v9.i7.98

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©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.

Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/

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Alkaline phosphatase staining. Cells were exposed to normoxia (21% O₂) for 14 d (NN) (A), normoxia for 7 d followed by hypoxia (5% O₂) for 7 d (NH) (B), hypoxia for 7 d followed by normoxia for 7 d (HN) (C), or hypoxia for 14 d (HH) (D) and then stained with alkaline phosphatase staining. The HN group showed the strongest and the broadest alkaline phosphatase staining (C).