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Real-time quantitative PCR of β-tricalcium phosphate discs wrapped with osteogenic matrix cell sheet. Increased collagen type I mRNA expression was observed in the HN group exposed to hypoxia (5% O2) for 7 d followed by normoxia (21% O2) for 7 d, compared with the NN group exposed to normoxia for 14 d (A). A tendency for higher expression of osteocalcin mRNA was also observed in the HN group (B). aP < 0.05.
