Table 2. Comparison of the diagnostic sensitivity of the vacuolation profile, IHC, and sandwich CDI for the detection of PrP^Sc in different anatomical areas of fCJD brains.

	Vacuoles				IHC				CDI
	Positive*		Score†		Positive*		Score		Positive*		D–N, cpm × 10-5
Anatomical Area	n+	%	Mean	S.D.	n+	%	Mean	S.D.	n+	%	Mean	S.D.
Frontal cortex	2	100	20.0	14.1	2	100	1.0	0.7	2	100	37.0	8.7
Entorhinal area	1	50	2.5	3.5	2	100	1.5	0.7	2	100	16.0	4.5
Parietal cortex	2	100	10.0	0.0	2	100	1.5	0.7	2	100	35.0	17.0
Temporal cortex	2	100	10.0	2.8	2	100	2.0	0.0	2	100	16.0	6.3
Hippocampus	0	0	0.0	0.0	2	100	1.5	0.7	2	100	41.0	11.0
Amygdala	0	0	0.0	0.0	1	50	1.0	1.4	2	100	25.0	23.0
Occipital cortex	2	100	13.0	9.9	2	100	2.5	0.7	2	100	27.0	14.0
Sulcus cingulate	2	100	25.0	7.1	2	100	1.0	0.0	2	100	28.0	14.0
Gyrus dentate	0	0	0.0	0.0	0	0	0.0	0.0	2	100	5.0	3.8
Insula	0	0	0.0	0.0	1	50	0.5	0.7	2	100	35.0	7.2
Thalamus	2	100	25.0	7.1	2	100	2.5	0.7	2	100	23.0	16.0
Nucleus caudate	2	100	60.0	28.3	1	50	1.0	0.0	2	100	48.0	7.8
Globus pallidus	2	100	7.5	10.6	2	100	1.0	0.7	2	100	19.0	0.4
Putamen	2	100	35.0	7.1	1	50	0.5	0.7	2	100	37.0	1.6
Midbrain	1	50	3.5	5.0	1	50	0.5	0.7	2	100	37.0	15.0
Pons	0	0	0.0	0.0	1	50	0.5	0.7	2	100	3.2	1.7
Medulla	1	50	4.0	7.5	1	50	0.5	0.7	2	100	8.8	2.4
Cerebellum	1	50	5.0	7.1	1	50	1.5	2.2	2	100	25.0	21.0

Adjacent sections from two fCJD(E200K) patients were analyzed in parallel by all three methods.

^*n+, number of samples with positive diagnosis of two samples examined. Percentages are based on two samples

^†Vacuolation scores are the percentage area of gray matter occupied by vacuoles, determined by the visual estimation of a single rater (S.J.D.)