Figure 5.

An overlay of the structures of receptor-bound insulin, AsnB26-insulin, and insulin analog with a triazole linker between position B26 and B29. The crystal structure of the receptor-bound insulin (in cyan, from the PDB ID 4OGA) (42) was superimposed on the crystal structure of AsnB26-insulin (in purple, from the PDB ID 4UNG) (47) and on the NMR structure of an insulin analog (in yellow, from the PDB ID 2N2W) (23) with a triazole crosslink (the nitrogen atoms are depicted in blue) between positions B26 and B29. Only the B-chains are shown for better clarity.