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. 2017 Jul 26;7:6493. doi: 10.1038/s41598-017-07013-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression and Ago integration of miRNAs after blood feeding in An. gambiae. (a) Changes in miRNA levels after blood feeding in terms of total small RNA, Ago1-IP RNA and Ago2-IP RNA. Each line represents one miRNA at six time points. Lines in red: upregulated miRNAs; lines in blue: downregulated miRNAs; lines in gray: miRNAs with no significant change. (b) Heatmap shows the log2-fold changes in miRNAs relative to the non-blood-fed controls. A Pearson correlation analysis were performed to assess the relationship between miRNA abundance in the total small RNA and in the Ago-IP RNA. Cor., Pearson correlation coefficient.