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. 2017 Jul 26;7:6618. doi: 10.1038/s41598-017-07011-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HIF-1α and PKM2 expression was induced in skeletal muscle at 3 days after burn injury. Protein expression of HIF-1α, PKM2, and PKM1 was examined in skeletal muscle of naïve (Control) mice and burned mice at 6 h, 1, 3 and 7 days after burn. HIF-1α and PKM2 protein expression was significantly increased at 3 days after burn (A–C). Burn injury did not alter protein expression of PKM1 (D). **P < 0.01, ***P < 0.001. ns: not significant. n = 4 mice per group.