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. 2017 Jul 26;7:6554. doi: 10.1038/s41598-017-06914-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibitory effect of GSK-J4 on LPS-induced BV-2 microglial cells. BV-2 microglial cells were treated with different concentrations of GSK-J4 for 4-h, followed by treatment with LPS (10 ng/ml). Inflammatory genes were significantly down-regulated in cells treated with GSK-J4 compared to untreated cells (*P < 0.01 and **P < 0.001) at the indicated times. Gene expression was normalized to GAPDH transcript levels. The data represent three independent biological experiments. The values are the mean ± SD of triplicate wells.