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. 2017 Jul 26;7:6520. doi: 10.1038/s41598-017-04765-8

Figure 2.

Figure 2

Optimal concentration of compounds that enhance MMLV-RT tailing activity. (a) Tailing reactions were conducted in the presence of each compound at different concentrations. Results are shown as averages of triplicate experiments. For dA, rA, dG, and rG, concentrations are shown as the % saturated concentrations, which were measured in TE buffer at 25 °C and were 50 mM, 25 mM, 13 mM, and 2 mM, respectively. (b) Tailing index values were calculated from the results at the optimal concentration shown in (a).