Fig. 1.

Effects of ATP and other nucleotides on MgtE. a Isothermal titration calorimetry (ITC) data of MgtE with ATP and other nucleotides. The data were obtained from MgtE with ATP, ADP, GTP, and ATP+Mg²⁺. The raw ITC data and the plots of injected heat for 20 automatic injections of 10 mM nucleotide solution into the sample cell containing the MgtE solution are shown. Measurements were repeated twice, and similar results were obtained. b, c Protease protection of MgtE by Mg²⁺ and nucleotides. The numbers on the left side indicate the molecular masses (in kilodaltons) of the markers. The details of the cleavage sites are described in Supplementary Fig. 1