Fig. 3.

Overall structure and ATP binding site of MgtE. a Ribbon representation of the full-length MgtE structure in complex with ATP, viewed parallel to the membrane. The N domain, CBS domain, plug helix, and transmembrane helices in one subunit are colored blue, green, yellow, and red, respectively. ATP and Mg²⁺ are shown as sphere models a, c. b Close-up view of the ATP binding site in the full-length structure. The dotted black line represents the minimum distance between the β-phosphate group of ATP and Mg²⁺ (5.4 Å). ATP is shown as a stick model b, d. c Ribbon representation of the MgtE cytosolic domain structure in complex with ATP. d Close-up view of the ATP binding site in the MgtE cytosolic domain structure. Amino acid residues involved in ATP-binding are shown as stick models. Dotted black lines indicate the hydrogen bonds between MgtE and ATP