Fig. 4.

Biochemical analyses of the ATP-binding site mutants of MgtE. a Protease protection of the MgtE mutants by Mg²⁺ and ATP. Protease protection of wild type MgtE by Mg²⁺ and ATP is shown as a reference. The numbers on the left side indicate the molecular masses (in kilodaltons) of the markers. b ITC data of the F227A and R187E mutants with ATP. The raw ITC data and the plots of injected heat for 20 automatic injections of 10 mM nucleotide solution into the sample cell containing the MgtE solution are shown. Measurements were repeated twice, and similar results were obtained