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. 2017 Jul 26;7:6635. doi: 10.1038/s41598-017-06682-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Serum survival of K. pneumoniae strains. Strains were incubated in different concentrations of human serum (a) or 75% serum complement-inactivated by heat (HI) or cobra venom factor (CVF) treatment (b) for 3 h at 37 °C. The recovered bacterial count was expressed as percentage of the initial inoculum. ATCC43816 (O1:K2) and PCM-27 (O2:K27) strains were used as comparators.Graphs show combined results of 2 and 3 experiments for the active and heat inactivated serum experiments, respectively. The same O3 isolates were stained with 10% human serum pool and IgG and IgM antibodies binding to live bacteria were detected with a labelled anti-human IgG or IgM by flow cytometry (c). The median fluorescent intensity is shown as arbitrary units. The difference between the staining intensity for O3 and O3b strains was not significant using the Mann Whitney test (p > 0.05; n.s.). O-serotype and capsule type of the strains are indicated in Table 2.