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. 2017 Jul 26;7:6627. doi: 10.1038/s41598-017-06865-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Microfluidic ratchets to separate leukocytes directly from whole blood. (A) Image of the sorting region of microfluidic ratchets infused with food color dyes illustrating the diagonal trajectory of the sample inlet flow. (B–E) Images of the leukocytes stained with Hoechst 33342 being separated from the whole blood and traversing diagonally through the funnel matrix. (F,G) Bright field and fluorescence images of the outlets showing the separate reservoirs into which RBCs and leukocytes are collected.