Table 2.
Performances specifications of recent microfluidic research in leukocytes separation from whole blood using biophysical methods.
| Method* | Performance Metrics | ||||
|---|---|---|---|---|---|
| Leukocyte Separation Efficiency | Leukocyte Purity | RBC Depletion Rate | Throughput | Requirement for Blood Dilution | |
| Microfluidic Ratchet Current Study | 98–99% | 100% | 100% | 5 µL hour−1 | No |
| Magnetophoresis19 | n.d. | n.d | 93.5% | 5 µL hour−1 | Yes (1:10 in PBS) |
| Magnetophoresis20 | n.d. | n.d | 95% | 0.5–0.7 mL hour−1 | Yes (1:20 in PBS) |
| Magnetophoresis21 | n.d. | n.d | 93.7% | 0.12–0.92 µL min−1 | Yes (1:40 in PBS) |
| Dielectrophoresis (DEP)17 | 76.9–92.1% | n.d. | n.d. | 50 µL hour−1 | Yes (1:5 in PBS) |
| DLD10 | 99% | n.d | n.d. | 1 µL min−1 | No |
| Leukocyte margination14 | 80% | 90% | n.d. | 600 µL hour−1 | Yes (0.5–2% hematocrit) |
| Leukocyte margination15 | 97% | 100% | n.d. | 1 µL min−1 | Yes (1:1000 in PBS) |
| Leukocyte margination16 | 67% | 1% | n.d. | 1 µL hour−1 | No |
n.d. Data not available.
*References in uppercase.