Table 2.

QCM affinity measurements of oligomeric TNF muteins.

	Protein	EHD2-sc-mTNFR2	p53-sc-mTNFR2	GCN4-sc-mTNFR2	sc-mTNFR2
high density	Kon (M−1 s−1)	6.07 × 105	6.16 × 105	1.67 × 106	8.08 × 105
	Koff (s−1)	3.74 × 10−5	2.69 × 10−5	8.77 × 10−5	3.99 × 10−4
	Kd (nM)	0.0615	0.0437	0.0527	0.4494
low density	kon1 [M−1 s−1]	5.33 × 105	1.28 × 106	2.16 × 106	n/d
	Koff1 [s−1]	6.3 × 10−2	6.51 × 10−2	3.47 × 10−2	n/d
	Kd1 (nM)	118	50.9	16.1	n/d
	kon2 [M−1 s−1]	n/d	2.20 × 106	3.45 × 106	n/d
	Koff2 [s−1]	n/d	8.76 × 10−6	5.97 × 10−6	n/d
	Kd2 (nM)	n/d	3.97	1.73	n/d