Fig. 2.

LPS enhances IL-6 and MCP-1 mRNA expression in PDL but not HaCaT cells. The SLPI protein was determined in PDL and HaCaT cells by Western blotting (a). The PDL cells and HaCaT cells were cultured under identical culture conditions. The SLPI protein is detected at the expected molecular weight (~14 kDa). The relative concentration of SLPI protein was assessed by densitometry and normalized to GAPDH serving as internal control. PDL and HaCaT cells, cultured under identical conditions, were stimulated with LPS (1 µg/ml) for 24 h and IL-6 and MCP-1 mRNA expression determined by quantitative real-time RT-PCR (b, c). Values are presented as mean ± SEM of 3-6 observations in each group. * and *** represent P < 0.05 and P < 0.001, respectively. ns non-significant