Figure 2.

Identification of miRNAs that alter cell proliferation in HeLa cells. In 96-well plates, 8000 HeLa cells were reverse transfected with miRNA inhibitors (5 pmol) in triplicates using Ambion siPORT Neo-FX. Seventy-two hours post-transfection, cells were fixed with 4% paraformaldehyde, permeablized with 0.1% TritonX-100 and stained with propidium iodide to look at total cell number. The plates were scanned using the TTP LabTech Acumen Explorer.