Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 26;7:6577. doi: 10.1038/s41598-017-06028-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Microfabricated structures using GelMA/ND nanocomposite hydrogels and evaluation of their biocompatibility. (A) Schematic describing the process of micropatterning of the GelMA/ND prepolymer solution into nanocomposite hydrogel with a 1 mm diameter. (B) Brightfield images of 1 mm in diameter micropatterned hydrogels with (+ND) and without (−ND) NDs encapsulating hASCs in 3D after ten days of culture. (Scale bar = 1 mm). (C) Calcein-AM staining of hASCs cultured in GelMA/ND micropatterned hydrogels at different time points. hASCs proliferated and spread throughout the microfabricated constructs after ten days. (Each scale bar = 200 µm). (D) The prepolymer GelMA/ND solution can also be bioprinted into more complex structures as shown in the phase contrast image (scale bar = 1 mm). The calcein-AM staining (green) shows the hASCs that were seeded on the bioprinted nanocomposite GelMA/ND and cultured for five days. (Scale bar = 100 µm). (E) MTS assay of hASCs encapsulated in GelMA/ND nanocomposite hydrogels demonstrated excellent biocompatibility regardless of the ND concentration in the gel. Results are reported as mean ± S.D., (n = 5). No statistical difference was observed among the groups tested.