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. 2017 Jul 26;7:6577. doi: 10.1038/s41598-017-06028-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Assessment of osteogenic differentiation of hASCs by qPCR analysis. The fold expression increase of different genes have been evaluated including (A) RUNX2, (B) osteopontin SPP1, (C) alkaline phosphatase ALP and (D) osteocalcin BGLAP. β-actin ACTB has been used as control gene and data were calculated following the relative method ΔΔCt. Results are normalized based on the values obtained from the Ctrl (−) group at day 7. Groups tested are respectively hASCs cultured using GelMA hydrogels in basal media Ctrl (−), GelMA/ND 0.2% w/v nanocomposite scaffold without Dex in basal media (ND), GelMA hydrogels containing dexamethasone 1 µM in osteoconductive media (Dex), the nanocomposite system containing the complex in osteoconductive media (ND/Dex) and hASCs cultured in osteoinductive media Ctrl (+). Results are shown as mean ± S.D. (n = 3) (*p < 0.05 **p < 0.01, ***p < 0.001).