Table 1.
A list of primers used in this study.
| Gene | use | species | F/R | Sequence |
|---|---|---|---|---|
| Actb | RT-PCR | mouse | Forward | CTTTCTACAATGAGCTGCGTG |
| Reverse | TCATGAGGTAGTCTGTCAGG | |||
| Ap2 (Fabp4) | RT-PCR | mouse | Forward | AACACCGAGATTTCCTT |
| Reverse | ACACATTCCACCACCAG | |||
| Cebpa | RT-PCR | mouse | Forward | GCCTTCAACGACGAGTTCCT |
| Reverse | CTCCCGGGTAGTCAAAGTCA | |||
| Cebpb | RT-PCR | mouse | Forward | GCAAGAGCCGCGACAAG |
| Reverse | GGCTCGGGCAGCTGCTT | |||
| Cebpd | RT-PCR | mouse | Forward | TCGACTTCAGCGCCTACATTGACT |
| Reverse | CCGCTTTGTGGTTGCTGTTGAAGA | |||
| Glut1 | RT-PCR | mouse | Forward | CAGTTCGGCTATAACACCGGTGTC |
| Reverse | ATAGCGGTGGTTCCATGTTT | |||
| Hk2 | RT-PCR | mouse | Forward | CAACATCCTGATCGATTTCACA |
| Reverse | GCAGTCACTCTCGATCTGAGAC | |||
| Klf4 | RT-PCR | mouse | Forward | GCGGGAAGGGAGAAGACAC |
| Reverse | GGCTCGGGCAGCTGCTT | |||
| Klf5 | RT-PCR | mouse | Forward | GGTTGCACAAAAGTTTATAC |
| Reverse | GGCTTGGCGCCCGTGTGCTTCC | |||
| Pparg | RT-PCR | mouse | Forward | CCAGGCTTGCTGAACGTGAA |
| Reverse | GGGGAAGACGAGGATGAAGC | |||
| Vegf | RT-PCR | mouse | Forward | TTACTGCTGTACCTCCAC |
| Reverse | ACAGGACGGCTTGAAGATA | |||
| leptin | RT-PCR | mouse | Forward | GTGGTCGGAAGCCCTGAGATAG |
| Reverse | GGGCGATCACTCGATGGAA | |||
| gRNA_mHif1a437 | gRNA | mouse | Forward | CACCGTTTCTTCTCGTTCTCGCCGC |
| Reverse | AAACGCGGCGAGAACGAGAAGAAAC | |||
| gRNA_mHif1a940 | gRNA | mouse | Forward | CACCGAAGTGCACCCTAACAAGCCG |
| Reverse | AAACCGGCTTGTTAGGGTGCACTTC | |||
| mCEBPB3k | Reporter assay | mouse | Forward | GGCGGTACCTTAGCAACCATCACAGCCACAG |
| Reverse | GCCTCGAGCGGGAGGTTTATAAGGC | |||
| mCEBPB2k | Reporter assay | mouse | Forward | GGCGGTACCTGTGATCGCAAACAGT |
| Reverse | GCCTCGAGCGGGAGGTTTATAAGGC | |||
| mCEBPB1k | Reporter assay | mouse | Forward | GCCGGTACCTGACAGGTTGGCAGCT |
| Reverse | GCCTCGAGCGGGAGGTTTATAAGGC | |||
| mCEBPB0.5k | Reporter assay | mouse | Forward | GGCGGTACCTGGAGAGTTCTGCTTC |
| Reverse | GCCTCGAGCGGGAGGTTTATAAGGC |