Fig. 1.

Vector duo and transduction of human and rat synoviocytes. a The BV^CARHAdV5 binary complex can be visualize by electron microscopy. The HAdV5 vector with a icosahedral shape, is bound to BV^CAR, a rod-shaped baculovirus which has incorporated the CAR glycoproteins in its envelope. Scale bar, 100 nm. b The schematic representation shows the BV^CARHAdV5 binary complex, with HAdV5 bound to BV^CAR via the CAR glycoprotein at the baculoviral membrane. The baculoviral envelope glycoproteins Gp64 are localized at the head of the baculoviral particle. c Human and d rat fibroblast-like synoviocytes (FLS) non-treated (grey bars) or pre-treated (black bars) with the proinflammatory cytokines were transduced with HAdV5-GFP alone, or with the BV^CARHAdV5-GFP complex. The figures represent the percentage of GFP-positive FLS cells analysed by flow cytometry. The average of three independent experiments, each in triplicates are shown. Statistical analysis using one-way ANOVA test, ***P < 0.001, **P < 0.01, *P < 0.05