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. 2017 Jul 26;7:6640. doi: 10.1038/s41598-017-06794-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Shape and Cell Count of Stretched and Unstretched MSCs. Stretched and unstretched MSCs that adhered to the collagen type I sheets were stained with Calcein to illustrate MSC shape (A). Cell nuclei stained with DAPI (not shown) were counted to measure cell numbers (B). The axis of stretch was along the horizontal direction. Clear differences in MSC alignment can be seen in stretched vs. unstretched MSCs, and alignment was more pronounced in 2× than 1× stretched MSCs (A). More subtle differences in cell size and cell spreading were also present and quantified elsewhere. The number of MSCs across the experimental conditions tested was not statistically different. Error bars indicate standard deviation.