Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 26;7:6542. doi: 10.1038/s41598-017-06970-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Human R-MIPs can be used to recover ribosome-associated mRNAs from cellular extracts of closely related eukaryotic species. (a) Detection of the indicated mRNAs from the specified species in extracts, MIPs and NIPs. RT-PCR products were visualised on agarose gels shown to the left. LysA is a spiked-in control used for normalisation. Images of uncropped gels and blots are shown in the Supplementary Fig. S1. The chart to the right shows relative recovery of indicated mRNAs with MIPs or NIPs as compared to the input extract (100%). RNA was quantified by RT-qPCR with the ΔΔCt method and normalised to LysA (see Methods). Standard error of means (SEM) are shown as bars; H. sapiens, n = 5, Luc reporter (pGL3) in HeLa cells; C. aethiops, n = 6, GFP reporter in VK219 cells; M. musculus, n = 5, endogenous eEF2 mRNA in C8-D1A cells; S. cerevisiae, n = 3, actin mRNA in yeast. *P < 0.05, two-tailed homoscedastic student’s t-test. (b) Imprinting factor (mean ± SEM) related to specified species ordered according to the evolutionary distance from human. The dotted line marks an IF of 1, indicating no preferential binding to MIP compared to NIP.