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. 2017 Jul 26;7:6494. doi: 10.1038/s41598-017-06750-7

Figure 2.

Figure 2

JMJD3 and NF-κB are required for up-regulation of Notch1 expression in scratch-wounded keratinocytes. (a,b) Following the depletion (siJMJD3) or inactivation (GSK-J4) of JMJD3, HaCaT keratinocytes were scratched and expression level was determined by quantitative PCR and Western blotting. (c,d) Following depletion (sip65) or inactivation (BAY11-7085) of NF-κB, HaCaT keratinocytes were scratched and expression level was determined by quantitative PCR and Western blotting. (e) ChIP assay was performed in scratch-wounded HaCaT keratinocytes. The occupancy of each protein was determined with quantitative PCR at the Notch1 gene encompassing the NF-κB binding sites. ChIP using normal IgG was performed as a negative control. (f,g) The methylation level of H3K27 was measured by ChIP assay in scratch-wounded HaCaT keratinocytes treated with GSK-J4 or BAY11-7085.