Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 26;7:6494. doi: 10.1038/s41598-017-06750-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Notch1 regulates keratinocytes migration via RhoU and PLAU gene expression in scratch-wounded keratinocytes. (a) Depletion (siNotch1) or inactivation (DAPT) of Notch1 (b) Depletion (siJMJD3) or inactivation (GSK-J4) of JMJD3 (c) Depletion (sip65) or inactivation (BAY 11-7082) of NF-κB suppresses RhoU and PLAU gene activation in scratch-wounded HaCaT keratinocytes. Transcripts of Notch1, Hes1, RhoU, PLAU, and GAPDH were determined by quantitative PCR. (d) Inactivation of Notch1 or JMJD3 or NF-κB results in decreased filopodia-like protrusions (white arrows), increased focal adhesion and actin stress fibers in scratch-wounded HaCaT keratinocytes. To detect filopodia and actin stress fibers, cells were stained with phalloidin (red). Focal adhesion was identified by cell immunostaining with anti-vinculin antibody (green). Nuclei were identified using DAPI staining. Scale bar, 25 μm.