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. 2005 Mar 2;33(4):1323–1331. doi: 10.1093/nar/gki270

Figure 4.

Figure 4

Specificity and dominance of RGG-box mediated repression. (A) Effect of the RBD on single activation domains (EAD, VP16, E1a and CREB). (B) Effect of RBD on two activation domains (EAD and VP16) present together. JEG3 cells were transfected with plasmids expressing the proteins indicated (as described in Materials and Methods) together with the following reporter constructs: p(Δ− 71)SomCat (for activators containing the ATF1 DNA-binding domain); pBS2CAT (for activators containing the BSAP DNA-binding domain); pG5E4TCAT (for G4E1a and E1aR) and pG1E4TCAT (for VP16 and derivatives). CAT assays were performed 40 h post-transfection and a representative CAT assay is shown. Relative transcription activity is shown in parenthesis to the right of each protein tested. For single activation domains (A) activity in the absence of the RBD is set at 100. For the joint activity of VP16 and the EAD (B) the activity of G4VP16 is set at 100.