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. 2017 Jul 26;7:6538. doi: 10.1038/s41598-017-06431-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotype with Overexpression and Repression of OsFH15. (A) qRT-PCR analysis of OsFH15 expression pattern in various tissues. (B) SANGER sequencing chromatography showing the mutations in Osfh15 mutant. (C) qRT-PCR analysis of OsFH15 expression from 14 d-old seedling in wild-type and transgenic plants, UBQ5 as control. (D) Spikelets of WT, Cas9 #13, RNAi #4 and OE #11 before heading. Bar = 5 mm. (E) Grains of WT, Cas9 #13, RNAi #4 and OE #11. Bar = 1 cm. (F) to (I) Grain length (F), Grain width (G) and Grain thickness (H) were determined by vernier depth. 1,000-grain weight (I) was weighed. Data are represented as mean ± SEM (n ≥ 100 in F-H, n = 15 in I). *P < 0.05, **P < 0.01, Student’s t tests were used to generate P values.