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. 2017 Jul 26;7:6538. doi: 10.1038/s41598-017-06431-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AF and MT Organization in Wild-Type and Transgenic Cells. (A) to (D) AF organization was stained by Alexa Fluor 488-phalloidin, (A) WT, (B) Cas9 #13, (C) RNAi #4, (D) OE #11. (G) to (J) MTs were stained with tubulin antibody, (G) WT, (H) Cas9 #13, (I) RNAi #4, (J) OE #11. (A) to (D) and (G) to (J) The cells from the inner epidermal cells of lemmas. (E) and (K) Fluorescence intensities corresponding to the regions of cell middle part in (A) to (D) and (G) to (J), respectively. This cell middle part was marked in Supplemental Fig. 2C with white line. (F) and (L) Average AF and MT density were measured and binned for the same regions in (A) to (D) and (G) to (J), respectively. Bar in (A) to (D) and (G) to (J) = 10 μm. Data are represented as mean ± SEM (n ≥ 50 in F and L). *P < 0.05, **P < 0.01, Student’s t tests were used to generate P values.