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. 2017 Jul 26;7:6538. doi: 10.1038/s41598-017-06431-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FH1FH2 Nucleates Actin Polymerization. (A) Time course of actin polymerization in the presence of FH1FH2 monitored by pyrene fluorescence. Different concentrations of FH1FH2 were added to 2 μM actin (10% pyrene labeled) before initiation of polymerization. a.u., arbitrary units. (B) Nucleation efficiency of FH1FH2. (C) to (E) Fluorescence image of Oregon-green-actin polymerization in the absence and presence of FH1FH2. (C) Oregon-green-actin alone, (D) Oregon-green-actin plus 100 nM FH1FH2, (E) Oregon-green-actin plus 200 nM FH1FH2. Bar in (E) = 5 μm. (F) Time course of actin polymerization in the presence of FH1FH2 and/or OsPRF1. (G) Time course of actin polymerization in the presence of FH1FH2 and/or OsPRF2.