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. 2017 Jul 26;7:6538. doi: 10.1038/s41598-017-06431-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FH1FH2 Attaches to the AF Barbed End and Inhibits the Elongation. (A) Kinetics of elongation of the barbed-end of AFs in the presence of FH1FH2. Preformed AF seeds (0.8 μM) were incubated with various concentrations of FH1FH2 before addition of 1 μM pyrene-actin monomers. a.u., arbitrary units. (B) Average elongation rates of AFs with or without FH1FH2 on the barbed end at different concentrations. Data are represented as mean ± SEM, n ≥ 30, N ≥ 3. (C) Kinetics of AF depolymerization in the presence of FH1FH2. FH1FH2 was incubated with 5 μM AFs (60% pyrene-labeled) for 5 min before dilution of the solution 25-fold in G buffer.