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. 2005 Mar 1;33(4):1269–1279. doi: 10.1093/nar/gki274

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© The Author 2005. Published by Oxford University Press. All rights reserved

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ChIP analysis of proteins associated with the β1-tubulin gene in nonheat shocked cells. Cross-linked chromatin from nonheat shocked cells was subjected to immunoprecipitation with antisera against NELF-B, NELF-E, DSIF or GAGA factor and with corresponding preimmune sera. (A) Schematic of β1-tubulin gene showing regions that were amplified by PCR (not drawn to scale). (B) Representative results obtained from a ChIP analysis. (C) Quantification of results obtained from three independent ChIP analyses.