Figure 2.

Effect of cadmium on the DNA-binding activity of MSH2–MSH6. (a) MSH2–MSH6 was pre-incubated with Cd²⁺ at 4°C for 10 min, mixed with reaction mixture containing labeled G/T mispaired DNA and further incubated at 4°C for 15 min in 20 μl final volume. Gel was run with 10 μl of the mixture, and autoradiographed. Quantitation was carried out with a phosphorImager. (b) Comparison of the ATPase activity (white bars) and DNA mobility shift activity (black bars) of MSH2–MSH6 (80 nM) in the presence of 0–0.5 mM Cd²⁺. Activity is presented as a percentage of the activity of MSH2–MSH6 in the absence of Cd²⁺ taken as 100%. The average of three experiments for each concentration of Cd²⁺ is presented. Bars also include standard deviation.