Figure 3.

Effect of cadmium on the ATP hydrolysis and ATP-binding activities of MSH2–MSH6. MSH2–MSH6 was pre-incubated with Cd²⁺ (0–0.5 mM) at 4°C for 10 min. Aliquots were withdrawn and assayed for ATP hydrolysis (black bars) and ATP binding (white bars) as described in Materials and Methods. Results are expressed as a percentage of the activity of MSH2–MSH6 in the absence of Cd²⁺. For ATP hydrolysis 100% corresponds to 200 pmol ATP hydrolyzed, while for ATP binding, 100% of the activity corresponds to 2 pmol ATP bound. The average of three experiments for each concentration of Cd²⁺ is presented. Standard deviation is included.